Abstract
ABSTRACT Acetaldehyde, ethanol and methanol evolution was studied from soybean (Glycine max L. Merrill) and snap bean (Phaseolus vulgaris L.) seeds held at 12, 53 or 75% RH. Exogenous acetaldehyde or ethanol was applied in a gaseous phase at known concentrations to seeds in a closed container. The volatile compounds evolved were quantified by gas chromatography from the headspace or thermally derived from seed tissues. Exogenous application of acetaldehyde or ethanol resulted in enhanced ethanol or acetaldehyde evolution, respectively, at all relative humidity levels tested. The greatest conversion of acetaldehyde to ethanol was at 75% RH, while maximal conversion of ethanol to acetaldehyde occurred at 53% RH. Ethanol evolution was greater than acetaldehyde evolution from treated seeds, supporting the contention that alcohol dehydrogenase (ADH) was responsible for the conversion, and that ADH favors ethanol production. Methanol evolution was enhanced only in the headspace by application of either acetal-d...
Published Version
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