Abstract
Evolution of the Serendipitous Discovery of Macrophage–Lymphocyte Interactions
Highlights
The transformation of column-purified lymphocytes with nonspecific and specific antigenic stimuli by Oppenheim JJ, Leventhal BG, Hersh EM
Our chromosome analyses were frustrated by the failure of leukemic lymphocytes from chronic lymphocytic leukemia (CLL) patients to be activated to divide and develop metaphases that could be analyzed for chromosome breaks in response to a kidney bean extract known as phytohemagglutinin (PHA)
Since the peripheral blood (PB) of more advanced CLL patients contained high numbers of white blood cells (WBC) consisting entirely of lymphocytes, we decided as a control to purify the non-adherent lymphocytes present in normal PBWBC by eluting them off sterile glass bead or nylon fiber columns, which retained the adherent phagocytic neutrophils and monocytes
Summary
The transformation of column-purified lymphocytes with nonspecific and specific antigenic stimuli by Oppenheim JJ, Leventhal BG, Hersh EM. This contribution to the project on the Living History of Immunology concerning “The Transformation of Column-Purified Lymphocytes with Non-specific and Specific Antigenic Stimuli,” by Joost J.
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