Abstract
Experiments using the immunological technique of micro-complement fixation indicate that chipmunks diverged from the tree squirrel lineage during the Oligocene. Three chipmunk lineages (Tamias, Eutamias : Neotamias , and Eutamias : Eutamias ) have been distinct since the late Miocene. Fossil, morphological, karyotypic, and other immunological data are discussed and shown to agree with our conclusions on relationships of these three lineages.
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