Abstract
Publisher Summary To understand the evolution of strongly transforming, highly oncogenic retroviruses as an analogy for nonviral carcinogenesis, this chapter presents the study of reticuloendotheliosis virus T (REV-T), a highly oncogenic avian retrovirus with genus-specific relationships to mammalian C-type viruses. REV-T causes a rapid lethal lymphoma in young chickens. In culture, REV-T transforms spleen and bone marrow cells into the permanent lines of immature lymphoid cells. However, less than 0.01% of a bone marrow cell suspension is transformed. REV-T is structurally a recombinant between REV-A and specific sequences in turkey DNA (c-rel). To determine the retrovirus sequences that are needed to allow the formation of infectious virus in the presence of a helper virus, the deletions of spleen necrosis virus is done and the herpes simplex virus type-1 thymidine kinase gene is used as a marker for the formation of infectious virus. It has been found that all internal retrovirus coding sequences can be deleted without affecting the yield of virus. To determine the particular stage at which viral synthesis was blocked, the presence of progeny virions and formation of unintegrated viral DNA in cells infected with medium from transfected cells was studied in the chapter.
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