Abstract
Pichia pastoris, a methylotrophic yeast is a well-established heterologous expression system for production of recombinant proteins in industries on a large scale. Pichia pastoris is widely used microbial species in industries due to its unique features such as high cell density growth, streamlined downstream process and rapid expression that allows to yield high amount of proteins in short period of time. Pichia pastoris expression system is better equipped with promoters and markers. This enables better understanding the process of glycosylation that facilitate the process of production of recombinant proteins. The article will review on different types of proteins that are expressed in industries and focus on different types of vaccines produced using Pichia pastoris expression system. More specifically, the importance of Pichia pastoris expression system in development of vaccines by producing recombinant proteins for various diseases is studied. Several other aspects associated with Pichia pastoris expression system to produce a protein on large scale in healthcare industry are highlighted.
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