Abstract
Development of bioluminescence resonance energy transfer (BRET) based genetic sensors for sensing biological functions such as protein–protein interactions (PPIs) in vivo has a special value in measuring such dynamic events at their native environment. Since its inception in the late nineties, BRET related research has gained significant momentum in terms of adding versatility to the assay format and wider applicability where it has been suitably used. Beyond the scope of quantitative measurement of PPIs and protein dimerization, molecular imaging applications based on BRET assays have broadened its scope for screening pharmacologically important compounds by in vivo imaging as well. In this mini-review we focus on an in-depth analysis of engineered BRET systems developed and their successful application to cell-based assays as well as in vivo non-invasive imaging in live subjects.
Highlights
In the post-genomic era, rapid functional evaluation of protein– protein interactions (PPIs), protein phosphorylation, or protease function, which play a key role in various cellular processes such as signal transduction, cell division, transport, etc., in live cell condition is essential
Non-invasive imaging approaches such as bioluminescence resonance energy transfer (BRET) have been developed over the last decade, which allow the study of PPIs in their native environment and are capable of providing a unified platform that can be translated from cell culture-based assays to the imaging of live subjects [6, 7]
In this mini-review, we will be exploring some hitherto unexplained factors affecting the spectral pattern of several BRET systems and their successful application to cell-based assays as well as in vivo imaging of live subjects
Summary
In the post-genomic era, rapid functional evaluation of protein– protein interactions (PPIs), protein phosphorylation, or protease function, which play a key role in various cellular processes such as signal transduction, cell division, transport, etc., in live cell condition is essential. Non-invasive imaging approaches such as bioluminescence resonance energy transfer (BRET) have been developed over the last decade, which allow the study of PPIs in their native environment and are capable of providing a unified platform that can be translated from cell culture-based assays to the imaging of live subjects [6, 7].
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