Abstract
Pollinators are often perceived as a primary selective agent influencing flower traits such as colour, size, and nectar properties. The genus Fritillaria L. (Liliaceae), comprising approximately 150 species, is described as generally insect pollinated. However, there are at least three exceptions: two hummingbird-pollinated North American species and one passerine-pollinated Asian species. Despite this variation in pollination, little is known about flower traits that may accompany this shift in fritillaries. In this study, we aimed to assess the attractiveness of the floral traits for (new) pollinators and track the evolution of flowers traits in the context of a shift in the principal pollinator. Therefore, we studied 14 flower traits related to the pollination in 60 Fritillaria species and traced the evolutionary trajectory of these traits. We used a phylogenetic tree of the genus, based on five DNA markers (matK, rpl16, and rbcL, 18S, and ITS) to reconstruct the ancestral state of studied flower traits. The results show that in bird-pollinated species several new traits evolved. For example, flower colouration, nectar sugar, and amino acid concentration and composition fulfil the criteria of ornithophilous flowers, although flower traits do not exclude insect pollinators in bird-pollinated fritillaries. Interestingly, we recorded potential reversals from bird to insect pollination. Our analysis, showing a broad study of flower traits among closely related species in the context of pollinator shift, serves as a starting point for future work exploring the genetic and physiological mechanisms controlling flower traits in the genus Fritillaria.
Highlights
Angiosperms present enormous variation in flower traits, such as colour, shape, size, content and quality of the floral reward
The phylogeny analysis included in our study covered approximately 76% of the species currently recognised in the Fritillaria genus, with matK having the highest coverage (97%), and internal transcribed spacer (ITS) and 18S having the lowest coverage
The analysis resulted in a tree based on five sequences resolving the Fritillaria genus as monophyletic
Summary
Angiosperms present enormous variation in flower traits, such as colour, shape, size, content and quality of the floral reward. This variation is encountered among closely related species (e.g., Johnson et al, 1998; Wilson et al, 2004; Smith et al, 2007; Thomson and Wilson, 2008; Guzman et al, 2017; Roguz et al, 2018). Several empirical studies have supported the relation between flower traits and pollinators groups (Dellinger, 2020). This relation is even more visible, when pollinators shifts are considered
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