Evoluation of HBV Antibodies Evoked by Immunization And Infection Among Health Employee and Population

Abstract

In present study HBV-markers (HBs Ag and Anti HBs) were investigated in two different groups. HBV antibodies evoked by immunization and infection in health employees and population were researched and compared each other. From January 1998 to January 2000 we investigated hepatitis B virus markers (HBS Ag and Anti HBs) by ELISA technique (Bioelisa, Biokit S.A., Spain) in total of 248 persons, 100 from normal population and 148 from healthy hospital employees. Following detection of HBV markers, HBV vaccine (GenHevac B, Pasteur) was administered in 96 subjects with being seronegative of HBs Ag and Anti HBs. Serum samples were collected on the 30th days of postvaccination for Anti HBs response. In the group of healthy hospital employees, the frequency of positivity for HBs Ag and Anti HBs antibodies was 35.14 % (52 of 148) and 5.41 % (8 of 148) respectively. At the same time HBs Ag and Anti HBs positivity were 5 % (5 of 100) and 26 % (26 of 100) in healthy population. There weren't any immunization occur after the first dose of vaccination. One month after the second dose, 3 subjects of hospital employees and one subject of healthy population and one month after the third dose, almost all subjects (except one in hospital employees) were being seroprotected. Hepatitis B virus is a common viral agent; so vaccination has been seemed to be necessary in order to eradicate Hepatitis B infection. The vaccine should be applied at high risk groups especially in health workers before beginning working. In this study it has been shown that the first and second dose of vaccine were insufficient in developing Anti HBs antibodies. In conclusion three doses immunization course against HBV were necessary to elicit protective levels of Anti HBs antibodies.