Abstract
We describe the results obtained in studies of the impulse activity recorded from isolated neurons of the hippocampal CA1 area in the perforated patch-clamp configuration. Spiking was initiated by two modes of intracellular stimulation, namely by application of rectangular 2.5-sec-long current pulses or a ramp protocol (linear current ramp within the 10.0-sec-long interval). We proposed schemes of recording allowing experimenters to obtain reliable quantitative characteristics of the activity of isolated neurons taking into account the problem of reproducibility of the results. We also examined the rhythmicity of generation of action potentials by the studied neuron and typical phenomena of its disturbances; the techniques for selection of an optimum mode of ramp stimulation of the neuron making it possible to obtain detailed characteristics of generation of impulse activity are also described.
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