Evidences reveal that cattle and buffalo evolutionary derived from the same ancestor based on cytogenetic and molecular markers

Abstract

Muscle-DNA from cattle and buffalo was extracted to amplify the mitochondrial DNA segment (cytochrome b gene) and the gene encoding species-specific repeat (SSR) region. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and SSR techniques were used to identify of species origin. Restriction analysis of PCR-RFLP of the mitochondrial cytochrome b segment and SSR analysis showed no differences between cattle and buffalo. Where, the fragment length (bp) generated by AluI PCR-RFLP were 190, 169 and PCR amplification size of the gene encoding SSR region was 603 bp in both cattle and buffalo. Consequently, finding from this study could be revealed that cattle and buffalo are evolutionary derived from the same ancestor.