Abstract
Abstract Pretreatment of sheep erythrocytes (E) with supernatants of short-term cultures of mouse spleen or thymus cells induces the following interactions with guinea pig complement: 1) Supernatant pretreated EAlim (S.EAlim) are less susceptible to lysis by excess complement. S.EAC1̄4 yield a lower C2 titer, have an increased Tmax, but an unchanged C2 decay when compared with medium-pretreated EAC1̄4. Lysis of already formed EAC1̄4̄2̄is not inhibited. Therefore the inhibition affects C2 activation. 2) E, pretreated with supernatant (S.E), are able to fix C1̄, as detected by transfer to EAC4: In order to generate the same number of C1̄-fixing sites by using IgM hemolysins, five to six times the optimal amount is required. Yet S.EC1̄ cannot be lysed by the other components added in excess. S.EC1̄ can remove C4 from the fluid phase with the same efficiency as EIgMC1̄ but S.EC1̄ treated with C4 cannot remove C2. 3) Native C1̄ binds to S.E: S.E and EIgM remove C1 from the fluid phase with the same efficiency; fixation of 1̄ on S.E is decreased after incubation with native C1̄
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