Abstract
When sporangia of Thraustochytrium aureum were grown in the presence of l -[4- 14 C]aspartic acid and [2- 14 C]acetate virtually no radioactivity was found in the lysine either free or bound in protein. No activity of saccharopine dehydrogenase (NAD + , lysine requiring) could be detected in extracts of the sporangia. These results indicate that neither the aminoadipic acid nor the diaminopimelic acid pathway are present in this organism. Attempts to confirm these findings by other growth studies using T. aureum and T. roseum were inconclusive. The results confirm previous investigations which appear to point to the conclusion that Thraustochytrium and related organisms are not fungi.
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