Evidence that renal arterial-venous oxygen shunting contributes to dynamic regulation of renal oxygenation

Abstract

Renal blood flow (RBF) can be reduced in rats and rabbits by up to 40% without significant changes in renal tissue Po(2). We determined whether this occurs because renal oxygen consumption changes with RBF or due to some other mechanism. The relationships between RBF and renal cortical and medullary tissue P(O(2)) and renal oxygen metabolism were determined in the denervated kidneys of anesthetized rabbits under hypoxic, normoxic, and hyperoxic conditions. During artificial ventilation with 21% oxygen (normoxia), RBF increased 32 +/- 8% during renal arterial infusion of acetylcholine and reduced 31 +/- 5% during ANG II infusion. Neither infusion significantly altered arterial pressure, tissue P(O(2)) in the renal cortex or medulla, nor renal oxygen consumption. However, fractional oxygen extraction fell as RBF increased and the ratio of oxygen consumption to sodium reabsorption increased during ANG II infusion. Ventilation with 10% oxygen (hypoxia) significantly reduced both cortical and medullary P(O(2)) (60-70%), whereas ventilation with 50% and 100% oxygen (hyperoxia) increased cortical and medullary P(O(2)) (by 62-298 and 30-56%, respectively). However, responses to altered RBF under hypoxic and hyperoxic conditions were similar to those under normoxic conditions. Thus renal tissue P(O(2)) was relatively independent of RBF within a physiological range (+/-30%). This was not due to RBF-dependent changes in renal oxygen consumption. The observation that fractional extraction of oxygen fell with increased RBF, yet renal parenchymal P(O(2)) remained unchanged, supports the hypothesis that preglomerular diffusional shunting of oxygen from arteries to veins increases with increasing RBF, and so contributes to dynamic regulation of intrarenal oxygenation.