Evidence that molecules influencing axonal growth and termination in the developing geniculocortical pathway are conserved between divergent mammalian species

Abstract

The general architecture of the visual system is similar for all species of mammal. To determine if the development of connections in the visual system might be under the influence of conserved molecules, we co-cultured explants of the murine lateral geniculate nucleus with slices from either murine or feline occipital cortex. Neurite outgrowth from embryonic murine geniculate explants was significantly enhanced by slices of newborn mouse occipital cortex or kitten visual cortex or by medium previously conditioned by these slices. Slices of similar volume but from sites other than occipital cortex had less or no effect on the murine geniculate explants. Fibers from murine geniculate explants grew freely on cortical slices from the kitten. They terminated mainly in layer 4 and also in layer 6, in both murine and feline visual and frontal cortical slices, irrespective of whether they entered through the white matter or pial side. Only the deep layers of the kitten's cortex sent projections to co-cultured murine geniculate explants. We suggest that the diffusible factors released by the cortex that stimulate the growth of axons from the lateral geniculate nucleus and the molecules that mark specific cortical laminae as targets for ingrowing afferents, are conserved in divergent species. We also found that murine geniculate axons grew freely on feline cerebellar slices. It is known from previous co-culture experiments that rodent geniculate axons are inhibited on rodent cerebellum and we suggest that the inhibitory factors involved are not conserved.