Abstract
Lysyl- and arginyl-tRNA synthetases have been found to exist in multiple molecular weight forms in rat liver. The small molecular weight forms of lysyl- and arginyl-tRNA synthetases copurify throughout a five step chromatographic procedure resulting in a purification of 370- and 140-fold, respectively. The enzymes appear to be homogeneous on Sephadex G-200 and elute at an apparent molecular weight of 240,000. Gas chromatography reveals that the synthetases contain nearly 14% carbohydrate by weight. The carbohydrates present are: mannose, fucose, glucose, galactose, N-acetylglucosamine and N-acetylgalactosamine. This is the first report that aminoacyl-tRNA synthetases may exist as glyco-proteins.
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