Abstract
We have studied the intracellular light chains of Pl, a mouse plasmacytoma derived from ADJ PC5, which secreted IgG2 a and excess light chains, and its mutant X 2Pl which secreted only light chains. In both cases the intracellular light chains were not covalently dimerised, carrying, as did the freshly secreted light chains, one free thiol group per polypeptide chain. The presence of a free thiol group intracellularly indicates that temporary blocking cannot play a significant role in the control of assembly of the L chain into IgG2 a. The light chains excreted in the urine of mice carrying these plasmacytomas have blocked thiol groups, and these results show that such blocking must occur extracellularly after secretion.
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