Evidence that intersegmental competition influences the segmental distribution of the central terminals of muscle sensory afferents in the chicken embryo

Abstract

The possibility that muscle sensory afferents that enter the spinal cord at different segmental levels compete for terminal space in the lateral motor column was tested by unilaterally ablating neural crest cells destined to generate dorsal root ganglia (DRGs). Newly migrating neural crest was surgically removed from several lumbosacral segments on one side of the spinal cord on day 3 of embryogenesis (d3). The resultant experimental preparations developed without DRGs on the operated side at the segmental levels where the ablation was performed. At d18 the central projection from the intact DRG immediately caudal to the operated segments was anatomically mapped with a lipophilic axonal tracer. The central projection from the same DRG on the opposite, unoperated side was mapped as a control. The density of terminals along the longitudinal axis of the lateral motor column was quantified in serial sections with an image analysis program. On both the operated and control sides, the majority of terminals were found within the segment of entry and the rostral and caudal neighboring spinal segments. However, the rostral neighboring segment received a larger proportion of terminals on the operated side compared to the control side. Thus, the absence of primary afferents entering the spinal cord at specific segments leads to a shift of the afferent projection to the lateral motor column from the neighboring segments towards the deafferented segments.