Abstract

A DNA probe specific for genes coding for the heat-labile toxin type II (LT-II), heat-stable toxin type Ib (ST-Ib) and Shiga-like toxin type II (SLT-II), were used to examine 118 fecal Escherichia coli strains isolated from healthy pigs. Fifty-six (47.4%) of the isolated were hybrid ized with the LT-II probe. Thirty-nine strains (33%) possessed ST-Ib genes and five of the 118 isolates (4.2%) showed homology with the SLT-II DNA probe. E. coli that possessed unusual toxin genes for pigs were isolated with a high frequency, which indicates the importance of toxigenic clones found in nature. Uncommon virulence factors should be examined in order to improve the efficiency of diagnosis and control procedures.

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