Abstract
A West Nile virus (WNV) surveillance network including sentinel chickens was deployed in Thessaloniki county, Greece, from May to July 2011. For the first time in summer 2011, a chicken WNV isolate from 6 July was molecularly identified. The partial NS3 sequence was identical to that of the Nea Santa-Greece-2010 WNV lineage 2, detected in central Macedonia in 2010. This suggests that WNV is actively circulating in central Macedonia and that it may have overwintered in northern Greece.
Highlights
A West Nile virus (WNV) surveillance network including sentinel chickens was deployed in Thessaloniki county, Greece, from May to July 2011
WNV lineage 2 was identified in two pools of Culex mosquitoes (Nea Santa-Greece-2010 virus) [2] and in wild birds [3] that were sampled during the epidemic season of 2010 from areas in close proximity to human cases
The sequence was deposited in GenBank database under accession number JN398476 and according to BLAST algorithm, it presented highest nucleotide sequence identity (99.73%) to that from Nea Santa-Greece-2010 virus derived from a Culex mosquito pool tested during the 2010 epidemic in Central Macedonia [7]
Summary
A West Nile virus (WNV) surveillance network including sentinel chickens was deployed in Thessaloniki county, Greece, from May to July 2011. The partial NS3 sequence was identical to that of the Nea Santa-Greece-2010 WNV lineage 2, detected in central Macedonia in 2010. WNV lineage 2 was identified in two pools of Culex mosquitoes (Nea Santa-Greece-2010 virus) [2] and in wild birds [3] that were sampled during the epidemic season of 2010 from areas in close proximity to human cases. A small scale mosquito and animal surveillance network was established in the county of Thessaloniki, one of the areas with the highest number of human cases during the epidemic of 2010 [1]. We report here preliminary findings of the study that will have interest for public health authorities
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