Abstract
<h3>Objectives</h3> To analyze the expression of biomarkers of cell proliferation and apoptosis in a murine model of sarcoma 180 (S180) treated with ethanolic extract of <i>Himatanthus bracteatus</i> (EEHB). <h3>Study Design</h3> S180 cells were transplanted to 30 mice treated with saline solution, 5-fluorouracil (5-FU), and EEHB (30 mg/kg) for 7 days. After euthanasia, tumor growth inhibition (TGI) and morphologic features of the tumors were assessed. Histochemical detection of in situ DNA fragmentation (TUNEL) and immunohistochemical expression of Ki-67, caspase-3, and cleaved caspase-3 were also analyzed. <h3>Results</h3> No significant difference was observed between the TGI in 5-FU (71.8 ± 8.1%) and EEHB (58.1 ± 6.0%; <i>P</i> > .05). Ki-67 expression was higher in saline (39.8 ± 2.3%) than in 5-FU (23.9 ± 1.0%; <i>P</i> < .005) and EEHB (24.2 ± 0.5%; <i>P</i> < .05), whereas TUNEL, caspase-3, and cleaved caspase-3 expressions were significantly lower in saline (6.1 ± 0.6, 1.3 ± 0.09, 4.8 ± 0.5, respectively) than in 5-FU (18.0 ± 1.5, 2.5 ± 0.06, 16.8 ± 0.8, respectively; <i>P</i> < .001) and EEHB (16.9 ± 1.3, 1.7 ± 0.06, 16.3 ± 0.4, respectively; <i>P</i> < .05). <h3>Conclusions</h3> The data suggest the involvement of the caspase-dependent apoptotic pathway in the antitumor activity promoted by EEHB.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
