EVIDENCE OF A TRANSIENT NATURE OF THE TH17 PHENOTYPE OF CD4+CD161+ T CELLS IN THE SYNOVIAL FLUID FROM PATIENTS WITH JUVENILE IDIOPATHIC ARTHRITIS.

Abstract

Event Abstract Back to Event EVIDENCE OF A TRANSIENT NATURE OF THE TH17 PHENOTYPE OF CD4+CD161+ T CELLS IN THE SYNOVIAL FLUID FROM PATIENTS WITH JUVENILE IDIOPATHIC ARTHRITIS. Laura Maggi1*, Lorenzo Cosmi1, Veronica Santarlasci1, Manuela Capone1, Valentina Querci1, Francesco Liotta1, Raffaele De Palma2, Rolando Cimaz3, Enrico Maggi1, Sergio Romagnani1 and Francesco Annunziato1 1 University of Florence, Department of Clinical and Experimental Medicine, Italy 2 Second University of Naples, Italy 3 Anna Meyer Children’s Hospital and University of Florence, Rheumatology unit, Italy OBJECTIVE: To investigate the phenotype and function of CD4+ T cells in synovial fluid (SF) from the affected joints of children with oligoarticular-onset juvenile idiopathic arthritis (JIA) and to establish a possible link with disease activity. METHODS: CD4+ T cells were obtained from the peripheral blood (PB) and SF of 23 children with oligoarticular-onset JIA, as well as from the PB of 15 healthy children and were analyzed for CXCR3, CCR6, CD161 expression and for IFN(interferon)- gamma and interleukin-17A (IL-17A) production. RESULTS: The numbers of CD4+CD161+ cells, showing either the type 1 T helper (Th1) or Th17/Th1 phenotype, were higher in SF than in PB of children with JIA. The few Th17 cells from SF of JIA underwent a spontaneous shift to the Th1 phenotype in vitro, whereas Th17 cells from PB of healthy children shifted only in presence of JIA SF; this effect was neutralized by antibody blocked of IL-12 activity. The frequencies of CD4+CD161+ cells, particularly the Th17/Th1 cells, in the JIA SF positively correlated with levels of erythrocyte sedimentation rate and C-reactive protein. CONCLUSION: These findings suggest that a shifting of CD4+CD161+ T cells from Th17 to the Th17/Th1 or Th1 phenotype can occur in the SF of oligoarticular-onset JIA, and indicate that the accumulation of these cells is correlated with parameters of inflammation. Thus, the results support the hypothesis that these cells may play a role in JIA disease activity. Keywords: Th17, Th1 Cells, CD161, JIA, RORC Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Maggi L, Cosmi L, Santarlasci V, Capone M, Querci V, Liotta F, De Palma R, Cimaz R, Maggi E, Romagnani S and Annunziato F (2013). EVIDENCE OF A TRANSIENT NATURE OF THE TH17 PHENOTYPE OF CD4+CD161+ T CELLS IN THE SYNOVIAL FLUID FROM PATIENTS WITH JUVENILE IDIOPATHIC ARTHRITIS.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00958 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Laura Maggi, University of Florence, Department of Clinical and Experimental Medicine, Florence, 50134, Italy, laura.maggi@unifi.it Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Laura Maggi Lorenzo Cosmi Veronica Santarlasci Manuela Capone Valentina Querci Francesco Liotta Raffaele De Palma Rolando Cimaz Enrico Maggi Sergio Romagnani Francesco Annunziato Google Laura Maggi Lorenzo Cosmi Veronica Santarlasci Manuela Capone Valentina Querci Francesco Liotta Raffaele De Palma Rolando Cimaz Enrico Maggi Sergio Romagnani Francesco Annunziato Google Scholar Laura Maggi Lorenzo Cosmi Veronica Santarlasci Manuela Capone Valentina Querci Francesco Liotta Raffaele De Palma Rolando Cimaz Enrico Maggi Sergio Romagnani Francesco Annunziato PubMed Laura Maggi Lorenzo Cosmi Veronica Santarlasci Manuela Capone Valentina Querci Francesco Liotta Raffaele De Palma Rolando Cimaz Enrico Maggi Sergio Romagnani Francesco Annunziato Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.