Abstract
Substrate isotope studies, solvent isotope studies, proton in-ventories and studies of Vmax and Km as a function of pH suggest an E1CB (V. E. Anderson (1991) in Enzyme Mechanisms for Isotope Effects (Cook, P. F., ed.), pp. 389–417, CRC Press, Boca Raton, FL) mechanism via an enolate intermediate for type II dehydroquinases involved in biosynthetic or catabolic pathways.
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