Abstract
Particles which have several biochemical and biophysical properties diagnostic of ribonucleic acid (RNA) tumor viruses of animals have been isolated from specimens of human milk. The human particles have a density of 1.16 to 1.19 Gm. per ml., a RNA-directed deoxyribonucleic acid polymerase, and a 60–70S RNA. The quantity of particles in particular milk samples is determined by hybridization of the polyadenylic acid regions of their 60–70S RNA to radioactive polyribouridylic acid of known specific activity. A method involving molecular hybridization with radioactive DNA complementary to the RNA of the mouse mammary tumor virus was used to detect homologous RNA in both murine and human malignant breast tumors.
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