Abstract
1. 1. We dissected, homogenized and prepared ganglia and connectives from the central nervous system of medicinal leeches for SDS gel electrophoresis. The isolated proteins were transferred to nitrocellulose and incubated with affinity column-purified antibodies. 2. 2. The immunoblots showed a strong positive reaction of a bovine carbonic anhydrase standard at a molecular weight of 29 kDa, and a distinct double-bond at the same molecular weight in the analyzed material. 3. 3. We demonstrated with rat antibodies that carbonic anhydrase II is detectable in the leech central nervous system as the main isoenzyme. 4. 4. The biochemical knowledge of carbonic anhydrase reported here agrees well with the immunocytochemical locations, thus affirming the validity of specific staining.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Comparative Biochemistry and Physiology -- Part B: Biochemistry and Molecular Biology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
