Abstract
Hydroxyurea, which brings about a rapid but reversible inhibition of DNA replication and histone synthesis in HeLa cells [l-7], has provided valuable information regarding the relationship between these metabolic events. Although time course and biochemical changes associated with inhibition have been extensively examined [l-7], little is known regarding the release from the metabolic block imposed by hydroxyurea. Because of the potential value of this inhibitor for further assessing the apparent functional coupling of DNA replication and histone synthesis, we have examined histone and DNA synthesis at various times after removal of hydroxyurea from HeLa cell cultures. These results indicate that the resumption of DNA synthesis precedes that of histone synthesis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
