Abstract
Histamine-containing cells in rats were identified by indirect immunofluorescent histochemistry using an antibody raised against histidine decarboxylase (HDC), the enzyme forming histamine, which was purified from fetal rat liver. HDC-like immunoreactive (HDCl) structures could be detected in the brain as well as in peritoneal mast cells and basal-granulated cells in deep crypts of the gastric mucosa of rats. Numerous HDCl neurons were found in the posterior hypothalamic area and HDCl nerve fibers with a varicose appearance of fluorescence were widely distributed in various regions of the brain.
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