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Abstract
Summary The aerobic reduction of nitro blue tetrazolium by saponified chlorophyll, irradiated with red light, was inhibited by the addition of superoxide dismutase. Removal of molecular oxygen also inhibited the reduction of NBT, but the addition of superoxide dismutase did not further depress this anaerobic NBT reduction. These observations point to a chlorophyll mediated photoproduction of superoxide in the presence of molecular oxygen. EDTA is not required in this reaction, as is the case when free flavins mediate the photoproduction of superoxide ions.
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