Abstract
Some large acidic oligosaccharides from bovine glycophorin were purified and their structures were determined by methylation analysis, nitrous acid deamination and selective acid hydrolysis after hydrazinolysis, Smith degradation and acetolysis. One oligosaccharide was a mixture of two decassacharides: ▪ ▪ Another acidic fraction was an undecasaccharide: ▪ The mixture of oligosaccharides released by NaOH-NaBH 4 treatment was subjected to selective acid hydrolysis after hydrazinolysis. The occurence of N-acetylglucosamine residue attached to C-6 of the reducing terminal N-acetylgalactosaminitol was demonstrated by isolating the dissccharide, GlcNAc(1→ 6)GalNAcol.
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