Evidence for the involvement of dopamine and 5-hydroxytryptamine in the regulation of the preovulatory release of luteinizing hormone in the domestic hen

Abstract

Injections of the dopamine (DA) precursor l-dopa and the 5-hydroxytryptamine (5-HT) precursor 5-hydroxytryptophan, beginning 6 hr before the predicted preovulatory release of LH, abolished the LH surge and blocked ovulation. The inhibitory effect of the drugs on the preovulatory release of LH was not reversed by injection of progesterone. This showed that the drugs inhibited LH release by acting on the central nervous system rather than peripherally, by inhibiting the release of progesterone from the ovary. The turnover rates of DA and 5-HT were estimated during the ovulatory cycle in the median eminence, the posterior hypothalamus, and the anterior hypothalamus. The turnover rates of the two monoamines were estimated from their rates of accumulation after inhibiting their catabolism with the monoamine oxidase inhibitor pargyline. Turnover of DA and 5-HT in the median eminence was depressed during the preovulatory release of LH and decrease in the anterior hypothalamus when preovulatory LH levels were increasing. Observations were also made of changes in the concentrations of hypothalamic DA, 5-HT, noradrenaline (NA), and adrenaline (A) during the ovulatory cycle. Concentrations of DA, 5-HT, and NA increased in the anterior hypothalamus and decreased in the posterior hypothalamus during the 4hr before the peak of the preovulatory release of LH. These results strengthen the view that hypothalamic DA and 5-HT play a role in the regulation of the preovulatory release of LH by a reduction in inhibitory inputs on LHRH neurones.