Abstract
A specific radioimmunoassay (RIA) has been developed for the quantitative measurement of the cAMP content of floral organs of Lilium cv. Connecticut. Significant quantities of cAMP were detected in and around the stigma (highest specific activity), style, ovaries and anthers, but not in the tepals or filaments. The specificity of the method was established by treating the extract of the upper part of the style (including the stigma) with bovine spleen phosphodiesterase. This led to a 50% loss of the cAMP content of the extract. Variations in the cAMP content of this organ were also observed 30 min after flower self-pollination and 90 min after tepal wounding. The presence of cAMP in the lily flower was confirmed by measuring the adenylyl cyclase activity in the membrane fraction of an extract of the upper part of a style (including the stigma). This activity was stimulated by forskolin and aluminium fluoride salts.
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