Abstract

BackgroundLinear motifs are short modules of protein sequences that play a crucial role in mediating and regulating many protein–protein interactions. The function of linear motifs strongly depends on the context, e.g. functional instances mainly occur inside flexible regions that are accessible for interaction. Sometimes linear motifs appear as isolated islands of conservation in multiple sequence alignments. However, they also occur in larger blocks of sequence conservation, suggesting an active role for the neighbouring amino acids.ResultsThe evolution of regions flanking 116 functional linear motif instances was studied. The conservation of the amino acid sequence and order/disorder tendency of those regions was related to presence/absence of the instance. For the majority of the analysed instances, the pairs of sequences conserving the linear motif were also observed to maintain a similar local structural tendency and/or to have higher local sequence conservation when compared to pairs of sequences where one is missing the linear motif. Furthermore, those instances have a higher chance to co–evolve with the neighbouring residues in comparison to the distant ones. Those findings are supported by examples where the regulation of the linear motif–mediated interaction has been shown to depend on the modifications (e.g. phosphorylation) at neighbouring positions or is thought to benefit from the binding versatility of disordered regions.ConclusionThe results suggest that flanking regions are relevant for linear motif–mediated interactions, both at the structural and sequence level. More interestingly, they indicate that the prediction of linear motif instances can be enriched with contextual information by performing a sequence analysis similar to the one presented here. This can facilitate the understanding of the role of these predicted instances in determining the protein function inside the broader context of the cellular network where they arise.

Highlights

  • Linear motifs (LMs) are short stretches of amino acids that populate protein sequences and play fundamental roles in protein interaction networks [1]

  • LMs frequently show wide variation in residue conservation: some positions accept only one or few amino acids while others do not have any preference and function as spacers [4]. These sequence features give to LMs an evolutionary plasticity and an important role in the evolution of cellular networks by the addition of new functionality to proteins [1]

  • For the majority of the studied instances, conservation of the local amino acid sequence and/or the local structural tendency was found to be differentially distributed between sequence pairs with and without the motif. These findings are supported by examples where the regulation of the LM mediated interaction has been shown to depend on the modifications at neighbouring positions or is thought to benefit from the binding versatility of disordered regions

Read more

Summary

Introduction

Linear motifs (LMs) are short stretches of amino acids that populate protein sequences and play fundamental roles in protein interaction networks [1]. LMs frequently show wide variation in residue conservation: some positions accept only one or few amino acids while others do not have any preference and function as spacers [4]. These sequence features give to LMs an evolutionary plasticity and an important role in the evolution of cellular networks by the addition of new functionality to proteins [1]. They occur in larger blocks of sequence conservation, suggesting an active role for the neighbouring amino acids

Methods
Results
Discussion
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call