Abstract
BY the application of a wide variety of restriction enzymes and of recently developed DNA sequencing techniques, large segments of the SV40 genome have now been sequenced1,2. As a result, the expression of the viral genetic information can be studied in molecular detail. The major SV40-specific messenger RNA is a polyA-containing, cytoplasmic RNA which sediments at 16S and is only expressed after the onset of DNA replication3. In vitro translation studies have shown that it codes for VP1, the main structural protein of the virion (ref. 4 and A. Smith, personal communication). 16S mRNA hybridises mainly to the SV40 DNA Hind II + III restriction fragments K, F, J and G, which correspond to the region 0.95 to 0.17 on the standard map5,6. The localisation of these restriction fragments and of 16S mRNA is illustrated in Fig. 1.
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