Abstract
The influence of Pt(II) compounds on the 31P NMR spectra of natural DNA, synthetic polynucleotides, and nucleosomes was investigated. With Pt complexes which are anti-tumor agents, a new peak or shoulder centered at approximately 1.2 ppm downfield from the untreated DNA signal was observed. When Pt compounds known not to be anti-tumor agents were studied, no such new signal was observed. The most reasonable explanation for the downfield resonance is that it is a consequence of a structural change induced in the DNA by the anti-tumor agent. Since the effect of the Pt compounds on nucleosomes was similar, the same structural change is probably occurring in DNA in solution and in nucleosomes. A nonalternating dG . dC polymer, but not alternating G . C or any A . T polymers, exhibited a similar spectral change and this finding suggests that the structural change in the DNA arises primarily from reaction of Pt anti-tumor agents with adjacent G residues.
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