Abstract
Chinese hamster ovary cells were given two 450-rad doses of X rays separated by 1 or 2 h, during which the cells were held at room temperature to minimize progression through the cell cycle. The frequency of induced mutations to 6-thioguanine resistance was compared with that of mutations induced by a single dose of 450 or 900 rad. Mutation frequencies induced by the split dose were intermediate between those induced by the two single doses, suggesting that premutational lesions were repaired.
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