Abstract
It has been investigated if photoreduction of NADP+ (detected by absorption spectroscopy) may take place in lysed plastids within minutes of exposure to light. This process was made possible by removal of oxidants from the reaction mixture. Lysed plastids were suspended in a medium containing NADP+ , catalase, glucose oxidase and glucose; exposure to light simultaneously to addition of ferredoxin and ferredoxin-NADP+ reductase, resulted in increased absorbance in the band around 340 n m , while addition of 3-phosphoglycerate resulted in the disappearance of this band. Ferredoxin and ferredoxin-NADP+ reductase are only effective when photoactivation takes place before their addition to the medium.
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