Abstract
BackgroundGrass carp (Ctenopharyngodon idellus, GC), as the highest-output fish in China, is economically important. The production of gynogenetic grass carp (GGC) will provide important germplasm resource for producing improved GC. At present, knowledge regarding the heterologous sperm DNA in gynogenetic offspring is little. Thus, revealing paternal DNA in GGC at the molecular level would be highly significant for fish genetic breeding.ResultIn this study, ultraviolet-treated sperm of koi carp (Cyprinus carpio haematopterus, KOC, 2n = 100), was used to activate the eggs of GC (2n = 48). Afterwards, cold shock (0–4 °C) was administered for 12 min to double the chromosomes, resulting in GGC. No significant difference (p > 0.05) was found between GGC and GC in appearance, erythrocytes size and chromosome numbers. However, at the molecular level, a specific microsatellite DNA fragment (MFW1-gynogenetic grass carp, MFW1-G) derived from the paternal parent KOC was found to be transmitted into GGC.ConclusionsFor the first time, this study provided an evidence at the molecular level that the DNA fragment derived from the paternal parent occurred in GGC. This finding is of great significance for fish genetic breeding.
Highlights
Grass carp (Ctenopharyngodon idellus, GC), as the highest-output fish in China, is economically important
For the first time, this study provided an evidence at the molecular level that the DNA fragment derived from the paternal parent occurred in gynogenetic grass carp (GGC)
The eggs were treated with cold shock (4 °C) for doubling the chromosomes, which developed into GGC (Fig. 1)
Summary
Grass carp (Ctenopharyngodon idellus, GC), as the highest-output fish in China, is economically important. The production of gynogenetic grass carp (GGC) will provide important germplasm resource for producing improved GC. Revealing paternal DNA in GGC at the molecular level would be highly significant for fish genetic breeding. Artificial gynogenesis usually uses ultraviolet (UV)-treated heterologous sperm to activate the eggs, the eggs are treated by cold shock or heat shock to double the chromosomes, resulting in gynogenetic progenies. Yi et al [1] found microchromosomes from the second-generation gynogenetic gibel carp (Carassius auratus gibelio), in which the gynogenetic gibel carp eggs were activated by UV-treated sperm of blunt-nose black bream (Megaloabrama amblycephala Yin), providing the evidence at the cellular level that the genetic material from heterogenous sperm had the effects on gynogenetic offspring. The newly improved gynogenetic grass carp (GGC) have been formed by cold treatment to double the chromosomes of the GC eggs activated by UV-treated KOC sperm
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
