Abstract
We employed helical phasing analysis to study DNA conformation in the absence and presence of PUT3, a member of the Zn 2 Cys 6 binuclear cluster family of transcription factors. Our data indicate that the PUT3 target site UAS 10 does not bend appreciably in the absence of PUT3(1–100), but bends towards the major groove by approximately 46 degrees in the presence of PUT3(1–100). We employed helical phasing analysis to study DNA conformation in the absence and presence of PUT3, a member of the Zn 2 Cys 6 binuclear cluster family of transcription factors. Our data indicate that the PUT3 UAS 10 target site does not bend appreximately 46 degrees in the presence of PUT3(1–100).
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