Abstract
Three cell lines of BHK21/WI-2 cells persistently infected with rubella virus were established. These persistently infected cells constantly released a small number of rubella virus particles. The released viruses were temperature-sensitive variants ( pi variants). The uninfected BHK21/WI-2 cells also released a presumed latent virus by culturing at 34° but not at 39.5°. This temperature-sensitive latent virus carries reverse transcriptase and DNA-dependent DNA polymerase activities. Infection of BHK21/WI-2 cells with the wild-type rubella virus at a nonpermissive temperature (37°) resulted in a concomitant release of the latent virus with rubella virus. When the antigenicity of the rubella pi variants was examined, anti-rubella rabbit serum inhibited the hemagglutinating activity of the variants and the wild-type virus and neutralized the plaque-forming ability of the wild type but not the variants. However, the pi variants propagated either in BHK21/WI-2 or in Vero cells were neutralized with antiserum against the latent virus, whereas the wild-type rubella virus was not affected by the same serum. Therefore it is concluded that the rubella variants are hybrids and antigenically mosaic with a latent virus of BHK21/WI-2 cells.
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