Evidence for greater production of colonic short-chain fatty acids in overweight than lean humans

Abstract

BackgroundShort-chain fatty acids (SCFA) are produced by colonic microbiota from dietary carbohydrates and proteins that reach the colon. It has been suggested that SCFA may promote obesity via increased colonic energy availability. Recent studies suggest obese humans have higher faecal SCFA than lean, but it is unclear if this difference is due to increased SCFA production or reduced absorption.ObjectivesTo compare rectal SCFA absorption, dietary intake and faecal microbial profile in lean (LN) versus overweight and obese (OWO) individuals.DesignEleven (11) LN and 11 OWO individuals completed a 3-day diet record, provided a fresh faecal sample and had SCFA absorption measured using the rectal dialysis bag method. The procedures were repeated after two weeks.ResultsAge-adjusted faecal SCFA concentration was significantly higher in OWO than LN (81.3 ± 7.4 vs. 64.1 ± 10.4 mmol/kg, P = 0.023). SCFA absorption (24.4 ± 0.8 vs 24.7 ± 1.2%, respectively, P =0.787) and dietary intakes were similar between the groups, except for a higher fat intake in OWO. However, fat intake did not correlate with SCFAs or bacterial abundance. OWO had higher relative Firmicutes abundance (83.1 ± 4.1 vs 69.5 ± 5.8%, respectively, P = 0.008) and a higher Firmicutes:Bacteriodetes ratio (P = 0.023) than LN. There was a positive correlation between Firmicutes and faecal SCFA within the whole group (r =0.507, P =0.044), with a stronger correlation after adjusting for available carbohydrate (r = 0.615, P =0.005).ConclusionsThe higher faecal SCFA in OWO subjects is not due to differences in SCFA absorption or diet. Our results are consistent with the hypothesis that OWO subjects produce more colonic SCFA than LN due to differences in colonic microbiota. However, further studies are needed to prove this.