Abstract
Summary Treatment of intact cells from the glucocorticoid-responsive AtT-20/D-1 cell line with neuraminidase or phospholipase A 2 caused a reduction in the ability of these cells to incorporate labeled glucocorticoids. Similar treatments with proteolytic enzymes had no effect on steroid uptake. In contrast, cell-free binding to the cytosol receptor was not affected by neuraminidase or phospholipase A 2 treatment but was abolished by treatment with proteolytic enzymes indicating that the effect on cell uptake was not secondary to an effect on the cytosol receptor. These data suggest that steroid entry into the AtT-20/D-1 cell is mediated by components of the cell membrane which are sensitive to the loss of sialic acid groups or disruption of membrane architecture.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
