Abstract
Applications of microwave spectroscopy as an index of the presence of free radicals in specimens of biological materials are discussed. The importance is stressed of this analytical tool in the search for mechanisms of the biologic action of radiation. Qualitative microwave spectroscopy is concerned with determining the type of free radical formed by irradiation and usually requires a detailed analysis of hyperfine structure. Many interesting results have been obtained by this method in investigating irradiated biochemicals such as amino acids, proteins, and nucleic acids. Quantitative microwave spectroscopy yields information on the number or concentration of free radicals as a function of radiation dose and dose rate, of time after irradiation, and of external conditions such as temperature and water content of the specimen or type and pressure of the surrounding gas. This method was applied to biochemicals and also to living cells exposed to ionizing radiation. Details of the experimental technique are summarized. Results are presented from studies on irradiated glycine, irradiated barley embryos, and irradiated seeds with various water contents. 15 references. (C.H.)
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