Abstract
A bioassay was developed to investigate causes of grape replant problems under controlled conditions. Soils were collected from methyl bromide-fumigated and non-fumigated plots at a site cleared from a 65-year-old grape vineyard (Vitis vinifera cv. Thompson seedless) at Parlier, CA. Subsamples of the non-fumigated soil were either left non-treated, subjected to autoclaving (twice 45 min), or heating at 40, 50, 60, 70, 80 or 90 °C for 30 min. Subsequently, the samples were placed in 120-mL pots, planted with rooted hardwood grape cuttings (V. vinifera, cv. Carignane) and placed in a greenhouse or growth chamber. Three months after transplanting, vines from non-treated or 40 °C-treated soil had lower shoot weights and densities of healthy lateral roots than vines from the other treatments. Pythium spp. were isolated from 45 to 55% of the plated root segments from vines grown in non-treated, or soil that had been heated at 40 or 50 °C but were not detected in roots from soil given other treatments. Egg masses of root-knot nematode, Meloidogyne spp., were produced on roots from non-treated or heated at 40 °C soil, but no egg masses were detected on roots of the other treatments. In another test with the same soils, remnant roots from non-fumigated or pre-plant methyl bromide-fumigated soil were extracted and amended to non-fumigated soil, soil from fumigated field plots, soil fumigated in a small container, or autoclaved potting mix. The transfer of old vine roots from non-fumigated field soil resulted in incidence of Pythium spp. on grape assay roots, but there was no measurable effect of the transfer on growth and health of the bioassay plant roots. The results of the bioassays indicate that grape replant problem at the California site had biological causes. The bioassay approach may aid in future determinations of the etiology of grape replant problems.
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