Abstract
The availability of procoagulant activities in platelet suspensions lysed by sonication correlated directly with the degree of lysis (loss of LDH and 51Cr). Separate measurements of platelet factor 3 (PF3) and Factor V/Va activity revealed significant differences in appearance of these platelet activities in sonicated cell suspensions. Platelet factor 3 activity increased almost in parallel to the loss of 51Cr, whereas Factor V/Va activity became available at a significant slower rate. This might be explained by the localization of Factor V/Va activities inside the α-granules and therefore not immediately available on the surface of the partially lysed platelets. No increase in lactate dehydrogenase or 51Cr-loss was observed during 12 min incubation of platelet suspension with collagen or thrombin, whereas both PF3 and Factor V/Va activities increased. Thus, the high platelet factor 3 activity of 34% (lysed platelets 100%) measured in thrombin stimulated platelet suspensions probably represents “lysis-independent” activity.
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