Abstract
In an attempt to localize endogenous prolactin (PRL) in rat ventral prostate gland at the light microscope level, the following 4-step immunohistochemical reaction sequence was applied to fixed tissue sections: I) goat anti-monkey γ-globulin (AMγG), II) NIAMDD rabbit anti-rat PRL (APRL), III) goat anti-rabbit γ-globulin: peroxidase conjugate (AR-γG-per), and IV) chromogenic substrate. AMγG was required to block the nonspecific staining pattern produced by direct binding of AR-γG-per to the tissue and unmask that produced by APRL. APRL-dependent staining occurred in the Golgi region of ventral prostate epithelial cells. Golgi region staining was attenuated and eventually disappeared with increased APRL dilution and was not reproduced when normal rabbit serum or rabbit antisera to rat LH or rat TSH replaced APRL. However, absorption of APRL with highly purified NIAMDD rat PRL (1-1) neither attenuated nor eliminated Golgi region staining. This suggested tliat the APRL-dependent staining of rat ventral prosta...
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