Evidence for an enkephalinergic system in the nervous system of the pond snail, Lymnaea stagnalis

Abstract

Evidence for the presence of an enkephalinergic system in the ganglia of the pond snail, Lymnaea stagnalis, has been obtained with 3 experimental approaches. Scatchard analysis with [ 3H]etorphine reveals a monophasic high-affinity opiate binding site ( K d 2.3nM ) which is naloxone-sensitive. Immunocytochemical localization of Met- and Leu-enkephalin-like substances as well as α-MSH- and ACTH-like materials was demonstrated within specific populations of neurons. Substances with Met- and Leu-enkephalin and Met-enkephalin sulfoxide RIA reactiveness were detected also in HPLC fractions corresponding to the retention times of authentic enkephalin standards. Together, the results provide strong evidence for the presence of enkephalinergic mechanisms in the nervous system of Lymnaea stagnalis. Additionally, the report provides indirect evidence for the existence of a macromolecular opioid precursor. This enkephalinergc system shows striking similarities to opioid mechanisms found in vertebrates and bespeaks a common evolutionary origin.