Abstract
The mechanism of androgen's action on cartilage growth plate is unknown. The aim of this study was to determine if receptors for androgen exist in dog and child epiphyseal growth cartilage. Cartilages were removed by microsurgical technique, from male dog bones, and at autopsy of a male newborn. 3H-DHT binding activity was evaluated in cytoplasmic and crude nuclear fractions of dog and child growth cartilage. Specifically bound 3H-DHT was determined by incubations of cytoplasmic or nuclear extracts with 3H-DHT (1-30 nM) alone or in the presence of an excess of unlabelled DHT to correct for non-specific binding. 3H-DHT specifically bound is 2 times higher in nuclear than in cytosolic fractions. Competition analysis revealed a high specificity of the binding component for DHT. Sucrose density gradient analysis of 3H-DHT labelled cytosol or nucleus from dog or child cartilage yielded one peak of radioactivity in the 4-S region. The high affinity, apparent specificity for DHT, and sedimentation analysis fulfill several of the criteria ascribed to a We thus suggest that the macromolecular protein binding we describe in dog and child growth plate cartilage is an androgen receptor.
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