Abstract

Event Abstract Back to Event Evidence for a rhodopsin-retinochrome photosensitive system in chromatophores of the squid, Loligo pealeii Alexandra Kingston1*, George Bell2, Alan M. Kuzirian2, Roger T. Hanlon2 and Thomas W. Cronin1 1 University of Maryland Baltimore County, Biological Sciences, United States 2 Marine Biological Laboratory, Marine Resources Center, United States Cephalopods, including squid, cuttlefish, and octopus, are hypothesized to have extraocular photoreceptors located in their skin. These putative photoreceptors are thought to detect light using an opsin-based photoreception system. In photoreceptors of the retina, an opsin protein is bound to a retinal chromophore to form a functional photopigment, and retinochrome acts as a photoisomerase, recycling the chromophore. The presence of opsin and retinochrome in close proximity suggests the presence of a functional photoreceptor. My work focuses on detecting opsin and retinochrome in putative extraocular photoreceptors in the skin of the longfin squid, Loligo pealeii. We searched for opsin and retinochrome in the skin and retinas of L. pealeii using RT-PCR. Opsin was detected in the retina, ventral mantle, ventral fin surface, 1st arm, 2nd arm, 3rd arm, 4th arm, tentacle and muscle tissue of L. pealeii. Retinochrome was detected in all these locations as well as the dorsal mantle and dorsal fin surface. Identical opsin and retinochrome transcripts were also detected in dissociated chromatophores from several locations. Future work will include in situ hybridization and immunohistochemistry to determine in what cells opsin and retinochrome transcripts and proteins are expressed. Keywords: chromatophore, retinochrome, Rhodopsin Conference: Tenth International Congress of Neuroethology, College Park. Maryland USA, United States, 5 Aug - 10 Aug, 2012. Presentation Type: Poster (but consider for participant symposium and student poster award) Topic: Sensory: Vision Citation: Kingston A, Bell G, Kuzirian AM, Hanlon RT and Cronin TW (2012). Evidence for a rhodopsin-retinochrome photosensitive system in chromatophores of the squid, Loligo pealeii. Conference Abstract: Tenth International Congress of Neuroethology. doi: 10.3389/conf.fnbeh.2012.27.00169 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Apr 2012; Published Online: 07 Jul 2012. * Correspondence: Ms. Alexandra Kingston, University of Maryland Baltimore County, Biological Sciences, Baltimore, MD, 21250, United States, acnahm@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Alexandra Kingston George Bell Alan M Kuzirian Roger T Hanlon Thomas W Cronin Google Alexandra Kingston George Bell Alan M Kuzirian Roger T Hanlon Thomas W Cronin Google Scholar Alexandra Kingston George Bell Alan M Kuzirian Roger T Hanlon Thomas W Cronin PubMed Alexandra Kingston George Bell Alan M Kuzirian Roger T Hanlon Thomas W Cronin Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

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