Abstract
Summary The kinetics of the incorporation of polymyxin into negatively charged phosphatidic acid bilayer vesicles has been investigated by a fluorescence polarization technique. Polymyxin is taken up within a second by the outer lipid layer of a vesicle. The incorporation time determined by the measurement of fluidity changes is independent of polymyxin concentration and of temperature in the range between 35° and 60°C. A diffusion controlled process has to be considered. The fluidity change after mixing the vesicles and polymyxin in solution exhibits a lag time period. This gives evidence for a cooperative binding process. The inner lipid layer of a vesicle is only affected at temperatures where the protein bound lipids are melted. A very slow transfer of polymyxin within 20 hours is observed.
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