Abstract
Three elicitor-active fractions were isolated from culture fluids of the rough isolate of Pseudomonas corrugata, a tomato pathogen that elicits the hypersensitive reaction (HR) in tobacco. Lyophilized, filtered culture fluids were extracted with methanol-ethyl acetate (80:20, vol/vol) to produce a water-soluble crude extract (CE). CE was partitioned with ethyl acetate to produce water-soluble aqueous (AQ) and organic (EA) fractions containing purified elicitor. P corrugata and the three fractions were tested for their ability to elicit HR in tobacco leaves, pith necrosis in tomato, and K + /H + exchange in tobacco tissue culture. Only P corrugata elicited pith necrosis in tomato; both bacteria and the fractions elicited HR in tobacco leaves; and bacteria and EA elicited K + /H + exchange in tobacco cells. The primary component in EA, HR2, did not cause a spreading lesion in tobacco and was therefore not a toxin. HR2 was found to be 95% pure by analytical high-performance liquid chromatography, to contain a fluorescent chromophore, and to be a peptide. HR2 contains glutamic acid, tyrosine, aspartic acid, glycine, alanine and/or arginine, isoleucine and/or leucine, and methionine and/or valine (approximate molar ratio 10:3:1:1:1:1:1). Since P corrugata is a useful biocontrol agent, these elicitors may have potential use in biocontrol.
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