Abstract
A fraction enriched in plasma membranes was isolated from rice roots by differential centrifugation and aqueous polymer two-phase partitioning. Analysis of the fraction by SDS-PAGE showed the presence of several low mobility polypeptides ( M r> 100 kDa). One of these polypeptides ( M r ~ 230 kDa) was specifically recognized by polyclonal antibodies to human erythrocyte spectrin. This finding suggests that a higher plant spectrin-based membrane skeleton may be preserved and studied using high-purity plasma membrane fractions obtained by aqueous polymer two-phase partitioning.
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